1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. BD Accuri® C6 is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. You are viewing an Accuri C6 Flow Cytometer. BD LSRFortessa 1. Online Booking System 2. The BD CSampler Plus adds a minimal footprint to the BD Accuri C6 Plus, about three feet square for the pair, keeping your benchtop free for other uses. … endstream endobj 30 0 obj <>>> endobj 31 0 obj <> endobj 32 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/Properties<>>>/Rotate 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 33 0 obj <>stream Such wear includes scratches, scrapes, and general dirtiness. A compact optical design, fixed No. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. 0 69 0 obj <>stream A unique, non-pressurized, peristaltic pump system drives the fluidics. As with any fluorochrome used in a flow cytometric assay, the success of FP detection depends on the level of FP expression within the cells, the excitation wavelength used and the light filters placed in front of the fluorescence detectors. Item is sold AS IS. Includes all accessories shown in pictures. filter, and bottle filters. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. 653146, 653148, and 653147 Sample Preparation Volume measurements on the BD Accuri C6 are most accurate with cell concentrations between 1,000 and 5000000 cells/mL. The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. Standard fluorochrome in use are identical to FACS Calibur. Which fluorochromes are compatible with the cytometers? Note: This is not a full list of all fluorochromes that are compatible with the flow cytometers. Amnis ImageStreamX. C6 Flow Cytometer 1-year Maintenance Kit . Nos. A unique low-pressure pumping system drives the fluidics. %%EOF Easy to use - fixed alignment and pre-optimized detector settings. 661085). BD Accuri™ C6 Flow Cytometer Features: Equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. The BD Accuri C6 flow cytometer was switched on and allowed to warm up for 15 minutes before analyses were begun. Overall very good cosmetic condition. ... BD Accuri C6. Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. The BD Accuri C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. The running costs are very low as it doesn¹t use any proprietary sheath fluid or reagents. Rockefeller University A compact optical design, fixed alignment, and pre- Phase III+: The University is open for expanded research operations; only authorized personnel will be admitted on campus. 29 0 obj <> endobj 52 0 obj <>/Filter/FlateDecode/ID[<6D17E613E92D6948AEF4F9A1572DFD51>]/Index[29 41]/Info 28 0 R/Length 106/Prev 60895/Root 30 0 R/Size 70/Type/XRef/W[1 2 1]>>stream BD LSRFortessa X-20. The non-pressurized system of BD Accuri C6 supports any brand of sample tubes – 12 x 75 mm or smaller (including microcentrifuge tubes), made of polypropylene or polystyrene. -Ģ �r^�F)�?2��7�% �K��x4JR���E�Y��Wl������3� �;p�y[�AD= r Also the filters and lasers can be swapped out to suit your needs with easily available off-the-shelf substitutes (this was mentioned by one of the Accuri techs, I personally cannot verify this). Access to training videos presented by FCR staff are here. The ideal cell concentration h�bbd``b`:$� -�8�`�$XJA�H�H0'��U �.+ BD Accuri C6, C6 Plus, and BD FACSVia Cytometers Perform these procedures when starting up your instrument after an extended period. Came with note that it needs repair. Higher concentrations might result in inaccurate counting due to system saturation, so dilute samples if necessary. BD LSRFortessa 2. All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. You can also set custom speeds/core sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. BD Accuri™ C6 software was started. The latest science discoveries delivered monthly to your inbox. Accuri C6 is a digital PC based bench top analyser, 2 laser system. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Meet the scientific leaders who are changing medicine, Peek inside our 70 biomedical laboratories, Learn more about our flexible, supportive academic programs, Learn about the breakthroughs happening every day, Hear from the world’s leading speakers and thinkers, We’ve spent 119 years perfecting the bioscience institute. Performance validation of the BD Accuri C6 … Filter and Fluorochrome List for the Accuri® C6 Flow Cytometer® System ©January, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP Cat. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. It is also supplied with a 24-tube rack for the walk-away acquisition of standard 12 x 75-mm tubes. With minor signs of normal wear and tear. To view hidden file extensions which distinguish BD Accuri C6 Software files (.c6) from BD Accuri C6 Software template files (.c6t): 1. 3. Available to trained users with online booking. 1230 York Avenue, Box 344 Brand BD Accuri™ Contents Contains (12) Peristaltic Pump Tubing, (6) Sheath Bottle Filters, (12) Decon/Cleaner Bottle Filters and (6) In-Line Sheath Filters In addition, the system derives sample volume and can calculate absolute counts or sample concentration per microliter. Hi, our Accuri C6 has stopped taking liquid. Brand: BD 653156 hެ�mS�8ǿ�^��[���L��@H�]�LC�w���$J�c����O�R�B ��:aGZ�V��6h���eZ3.ӆ !�N��Ӗ).�Θ����Y�2��V2#W�%㙱�(&���h&2�OäV 3��b#�(!5{���W�֕78���L�/��� Gը�_���~=ZU0�-a��aPW�Cw�����W8v4 �•���r���֍qlj�Į��!��e���N�f��p�c��ޟ-[ޟ�0��͵��I�~F�������ѭ3��w0�C�z�����?~������S�d��r��>U�j���~1���U#�^�&L�˥ӥM�����R)拷���. BD Accuri C6 Flow Cytometer Instrument Manual 7820018 Rev-4 1 1 INTRODUCTION The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Prevents the growth of bacteria in the sheath fluid of the Accuri™ C6 Flow Cytometer. BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from 48- and 96-well plates and deep-well plates. Filter and Fluorochrome List for the Accuri C6 Flow Cytometer ® System As of: January 1, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP For any system, we recommend: • Check all fluidic tanks for contamination and replace all cubitainers as applicable. All rights reserved. ... optical filters optimized for the detection of FITC, PE, PerCP, and APC. filters. BD Accuri™ C6 default optics Detector FL1 FL2 FL3 FL4 Filter 533/30nm 585/40nm 670nm LP 675/25nm Fluorochrome FITC PE PerCP, PerCP-Cy5.5 APC Color Green Yellow/Orange Dark Red Red “3 blue 1 … A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration of over 5 x 106 cells per mL. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. ... filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Analytical Flow Cytometry - Accuri C6 Configuration Please note that the Accuri C6 is for use by trained personnel only! h�b``�```�����`��π ���@q��L�n\g���`��h 'c�. endstream endobj startxref - Find MSDS or SDS, a COA, data sheets and more information. BD offers a selction of inferior vena cava filter products. ... considering that we still have to buy the replacement kit with filters, inline filter, and rubber tubbings. Packaged in a pack of ten 8mL vials. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Data is digitally collected over a 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. New York, NY 10065. Clinical Research and the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience Science, the Arts, and Culture. Millex Sterile syringe filter units are available in a larger 33mm housing with either MCE, PVDF or PES membranes. BD™ Accuri™ Bacteriostatic Concentrate Solution can be used to treat 10L of sheath fluid. Waste and sheath fluid bottles were checked during this time, and emptied or refilled as required. Since yeasts are relatively large (I use an Accuri C6 to count bacteria so yeast are large to me) I would go with the faster/larger core size. A compact optical design. This item has been powered on, but no further testing. FCRC Waiting List Forms Training can be requested via PPMS. The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. Contact, Flow Cytometry Resource Center (FCRC) Click on “View” tab 4. Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available. Table of Contents. The Selectable Lasers Module allows operation of the system in two additional configurations (2-Blue 2-Red, and 4-Blue) and significantly expands the fluorochrome combinations that can be analyzed. The default configuration of the BD Accuri® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. The Accuri C6 cytometer in my lab has some problems, and we need some help. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible Select “Tools” from the menu bar. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. Even more colors to choose from with selectable lasers and filter replacement. Ē\�@BBH"v@��d� �Hz �H4�0012����H%�?�ݏ x�S • Replace all fluid filters … This Technical Note Copyright 2004—2020 The Rockefeller University. When we turn on, the system does not collect liquid as normally, in fact is completely silent (no peristaltic pump working). The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. Click on the Windows Start button and select Control Panel from the pop-up menu. %PDF-1.6 %���� the Accuri C6 Flow Cytometer System. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Arts, and APC in a larger 33mm housing with either MCE, PVDF PES... No further testing not need to adjust detector voltages and replace all cubitainers as applicable a larger housing... 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Pre-Optimized detector settings filter units are available with the Selectable Lasers Module Cat..., the Arts, and APC throughput and fully automated acquisition from 48- and 96-well plates and deep-well.! Validation of the Accuri™ C6 flow Cytometer in the sheath fluid of the BD Accuri C6 Please! Panel from the pop-up menu costs are very low as it doesn¹t use any sheath... An extended period x 75-mm tubes standardized fluorescence performance so that users do need... Is also supplied with a 24-tube rack for the detection of fluorochromes as. And more information pre-optimized detector settings to your inbox SAMPLER supports the throughput! Standardized fluorescence performance so that users do not need to adjust detector.., C6 Plus, and general dirtiness Check all fluidic tanks for contamination and all... As required fluidic tanks for contamination and replace all cubitainers as applicable stopped taking liquid volume! Or PES membranes present to you, maybe the best entry level flow Cytometer replace all as... Been powered on, but no further testing 24-tube rack for the walk-away acquisition of standard 12 75-mm! Of inferior vena cava filter products fluorochromes that are compatible with the flow cytometers offers performance simplicity! Bacteria in the sheath fluid bottles were checked during this time, and APC accuri c6 filters.