BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. ..Cell membrane potential detection assay Cells were cultivated in 96-well plates, 1 × 105 cells/well. For the D Accuri™ 6, use the PE channel (FL2) for the reporter and the APC channel (FL4) for beads classification. BD Accuri C6 portable without the need for realignment. If you have any questions regarding this matter, please see the following BD Accuri Frequently Asked Questions or contact BD Service Contracts Administration 1.877.232.8995, prompt 4. Many BD Accuri C6 customers can begin collecting and analyzing data with the help of a quick start guide. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. (The instrument has even proved its mettle on an arduous, months-long research voyage to Antarctica.15) BD Accuri™ C6 software makes operation intuitive for novice and proficient users alike. delphineupvld. A two-fold reduction in fluorescence intensity per cell should accompany each cell division. Status:RUO Cat No.:661085. BD Accuri C6 with plate handler acquisition April 2016 Multi-well Auto Collect sample Important – only run this protocol after you have performed “Cytometer Setup/Verification” protocol on your BD Accuri C6 cytometer using particles. Cat No. Class 1 Laser Product. All Posts; Search. All current service contracts will be honored. The BD Accuri C6 Software installer is on the CD that came with the C6. easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. Results: A. Once the settings are optimized for your experiment, click Run to begin data acquisition. 21. Figure 4-3. The intuitive interface of the software guides the user through workflows. For Research Use Only. Data show overlays of single-parameter plots after fluorescence compensation has been applied. Please consult our cookie policy for more information. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Performance validation of the BD Accuri C6 … Article Title: Interaction of allogeneic adipose tissue-derived stromal cells and unstimulated immune cells in vitro: the impact of cell-to-cell contact and hypoxia in the local milieu Article Snippet: ..Flow cytometric analysis was performed using an Accuri C6 with BD Accuri C6 Software (BD Biosciences). BD Accuri™ C6 Plus Analysis Software for PC or Mac. https://www.biotechandmed.info/ Find the protocol for the sample preparation for cell cycle analysis in this website. Instructional video on the basics of using BYU's Accuri C6 cytometer. 22. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … Note: The linked templates on this page are provided for the convenience of our research customers. Installing BD Accuri C6 Software. Gate names are assigned sequentially by type (R1, R2 R3, P1, P2, P3, M1, M2 etc). Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. NOTE: If BD Accuri C6 Software .... BD Accuri™ C6 Plus software features an intuitive user interface. BD Accuri C6 software has an intuitive user interface that was developed based on hundreds of hours observing researchers using flow cytometers. • The Accuri is a volumetric injection system allowing for accurate information on volume collected and thereby allowing for cell count and concentration calculations. After contracts terminate, BD will support the BD Accuri C6 system for as long as reasonably possible, depending on parts availability and other matters. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Imaginatively, the associated software is called Accuri C6 software. Since a lot of questions are popping up on the group now, I thought I'd add my voice too. Waste and sheath fluid bottles were checked during this time, and emptied or refilled as required. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 10. Should updates in the values be required later, or if optimi-zation is needed, simply change the settings and re-analyze the data. Install BD CSampler software. Gate names are assigned sequentially by type (R1, R2 R3, P1, P2, P3, M1, M2 etc). Waste Line Quick Disconnect. A: After April 15, 2021, BD Accuri C6 instruments may obtain service from BD Biosciences depending on parts and personnel availability. 10. The BD Accuri C6 Flow Cytometer System The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Many BD Accuri C6 customers can begin collecting and analyzing data with the help of a quick start guide. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. The software still needs to be improved but it analysis with the FCS Express program is sufficient for our needs. Accuri C6 Software Imaginatively, the associated software is called Accuri C6 software. as created. BD Accuri™ C6 software was started. The Accuri system was engineered from the start to be compact, powerful and user-friendly, while also being affordable by most research laboratories. Cells were stained according to the kit procedure, acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. Finally, the Plant DNA Analysis Template was opened. This protocol also assumes a completed assay in a 96-well plate, with particles suspended in Run buffer. Quick View. Cited in over 9,000 publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. 11. $5,225.00 . 1.1 About This Manual This manual provides information on cytometer … The BD Accuri C6 flow cytometer was switched on and allowed to warm up for 15 minutes before analyses were begun. Figure 4-4. The templates have been designed using the current BD Accuri C6 software, version 264.21. BD Accuri™ C6 and BD Pharmingen™ Apoptosis, DNA Damage and Cell Proliferation Kit: BD Accuri™ C6 and BD Pharmingen™ Stem Cell Marker Antibodies, BD Accuri™ C6 and BD Stemflow™ hMSC Analysis Kit, BD Accuri™ C6 and BD Stemflow™ Human Definitive and Pancreatic Endoderm Analysis Kit, BD Accuri™ C6 and BD Stemflow™ Pluripotent Stem Cell Kits, Analysis of Cytokine Expression in Cancer Cell Lines Using Flow Cytometry, BD Apoptosis, DNA Damage and Cell Proliferation Kit and Template, BD™ Cytometric Bead Array (CBA) Reagents: A complete solution with the BD Accuri™ C6 Flow Cytometer, BD Horizon™ Fixable Viability Stain Reagents, BD Human Mesenchymal Stromal Cell Kit and Template, BD Induced Pluripotent Stem Cell Kit and Template, BD Intracellular T-Cell Kits and Templates, BD Mouse T-Cell Cytokine and Treg Kits and Templates, BD Naïve/Memory T- Cell Kits and Templates, BD Pluripotent Stem Cell Kits and Templates, Combining Cell Migration and Immunophenotype Assessment Using Flow Cytometry, Detecting Red Fluorescent Proteins on the BD Accuri™ C6, Dose–Response Studies Using Flow Cytometry, Immunophenotyping Cancer Cells Using Flow Cytometry, Protein Phosphorylation Analysis Using Flow Cytometry, Selectable Lasers Module for the BD Accuri™ C6 Flow Cytometer, BD Accuri™ C6 Software Quick Start Guide - Analyze, BD Accuri™ C6 Software Quick Start Guide - Batch Analysis, BD Accuri™ C6 Software Quick Start Guide - BD CSampler™ Accessory, BD Accuri™ C6 Software Quick Start Guide - Collect, BD Accuri™ C6 Software Quick Start Guide - Statistics, Analysis of 2102Ep Cells as a Pluripotent Stem Cell Reference Standard, A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples, Continuous Measurement of Intracellular Calcium, Determining Cell Concentration by Direct Volume, Identification of Human Peripheral Blood Cell Populations, Guide for Using BD™ Cytometric Bead Array (CBA) Kits, Guide for Using BD™ Cytometric Bead Array (CBA) Flex Sets, Guidelines for Journal Authors Using the BD Accuri™ C6, Multiparametric Analysis of Aquatic Organisms Using Flow Cytometry, Multiparametric Analysis of Microalgae for Biofuels Using Flow Cytometry, Optical Filter Guidelines for Fluorescent Protein Analysis, Platelet Counting with the BD Accuri™ C6 Flow Cytometer, Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants, Simplifying Complex Flow Cytometric Analyses, Simultaneous Analysis of GFP-Transgene Expression and Plant Cell Ploidy, Sowing the Seeds of Knowledge: The Integration of Practical Flow Cytometry into Undergraduate Education, Studying Cell Signaling with the BD Accuri™ C6 Flow Cytometer, Taking Advantage of Fluorescence Spillover to Analyze More Markers on Fewer Detectors: Thinking Outside the Orthogonal Box, The Personal Flow Cytometer in the Flow Cytometry Core Facility, Threshold and Analysis of Small Particles, BD Accuri™ C6 Flow Cytometer Optical Filter Guide, BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher, BD CSampler™ Accessory Installation Guide, Display of BD Accuri™ C6 Software-Generated FCS 3.0 Files Using FlowJo™ Version 8.8.7 or 9.4.11 for the Mac®, Guide to the FCS Express™ BD Accuri™ Software Importer, General preventative maintenance procedures, BD Naïve/Memory T-Cell Kits and Templates, BD Intracellular T-Cell Kits and Templates, BD Accuri™ C6 Eawag Water Quality Template, Annexin V FITC Apoptosis Detection Kit II, Apoptosis, DNA Damage and Cell Proliferation Kit, Mouse B-Lymphocyte Subset Antibody Cocktail, Mouse B-Lymphocyte Activation Antibody Cocktail, Mouse T-Lymphocyte Subset Antibody Cocktail, Mouse T-Lymphocyte Activation Antibody Cocktail, Human Pluripotent Stem Cell Sorting and Analysis Kit, Human Pluripotent Stem Cell Transcription Factor Analysis Kit, Human and Mouse Pluripotent Stem Cell Analysis Kit, Analyzing Data Collected on the BD Accuri™ C6 Using FlowJo™, Cell and Platelet Counting Using the BD Accuri™ C6 Flow Cytometer, Cell Biology Applications of Real-Time Flow Cytometry, Enabling a Deeper Understanding of What is on, in, and Made by the Cell, Flow Cytometry Applications in Cell Biology and Cancer Research, Flow Cytometry as a Tool for Microbial Analysis, Integrating Flow Cytometry into Education. They are not compatible with version 172.9, 227.4, or 202.8 software. The BD Accuri C6 Software Importer allows you to import your entire experiment from Accuri C6 and C6 Plus systems. BD Accuri C6 Flow Cytometry system available for sale. For information and special offers on the new BD Accuri C6 Plus personal flow cytometer, please visit our page. In-Line Sheath Filter. Digital signal processing and color compensation at any time BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. :661085, Status:RUO A: BD Accuri C6 training and applications support at customer sites will be offered based on the availability of staff with product knowledge. Please review our cookies policy and configure your cookies for your experience. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. © 2020 BD. Maintenance. The intuitive interface of the software guides the user through workflows. … The Accuri system was engineered from the start to be compact, powerful and user-friendly, while also being affordable by most research laboratories. They are not compatible with version 172.9, 227.4, or 202.8 software. 17. Hi, our Accuri C6 has stopped taking liquid. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. of the BD Accuri C6 also make it a valuable personal use tool for experienced researchers who want a cytometer to be easily available when and where they need it. 1. BD Accuri C6 Software automatically displays the percentage of cells within the region. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. All rights reserved. I've recently started doing flow cytometry (BD Accuri C6) in a core facility at my university, but my … Automated Preventive Maintenance Notification Window. Improper Shut Down Message. Samples were collected on the BD Accuri C6 flow cytometer using the kit template and analyzed using BD Accuri C6 software. This technical note describes how to access and change FlowJo axes settings to recreate plots zoomed in BD Accuri C6 software. Using the Validation beads provided by Accuri, run 50,000 events from each tube on the Slow setting and save the data as Today’s Date - Bead File. BD Accuri™ Remote Interfacing Module for BD Accuri C6 … Free software to analyze flow cytometry data? Run validation beads to ensure correct operation. Setup Beads 1: FITC Beads and 2: PE Beads Hi! BD Accuri C6 Software Menus .....75 Figure B-1. BD Accuri™ C6 software was started. Even new flow cytometry users find it so easy to learn and use that Figure Legend Snippet: Analysis of FRα expression on human PDAC cell lines AsPC-1, Capan-2, MIA PaCa-2, PANC-1 PDAC2, PDAC3, and PDAC5 Cells were stained with either APC- or PE- conjugated FRα-specific antibodies. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. This manual provides information on cytometer requirements and specifications, setup, and maintenance. Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus 19. In BD Accuri™ C6 software, the range of axes viewed is set using the Zoom Tool or the Plot Spec box. Class 1 Laser Product. 9. The Setup Beads 3: Raw Beads are used to setup the machine. 8. Service for BD Accuri C6 systems and their related options will … Figure 10 www.AccuriCytometers.com For Research Use Only. With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. I've recently started doing flow cytometry (BD Accuri C6) in a core facility at my university, but my … Q: How will we obtain service after April 15, 2021? A: After April 15, 2021, our sale of BD Accuri C6 spare parts and consumables will be dictated by parts availability and other restrictions. Set Core Size .....78. Download Bd Accuri C6 Software User Guide - google manual for iPad. • Digital Compensation- The Accuri C6 Plus software does not come with an automatic digital Quick View. $600.00 . If you click accept cookies then all cookies will be written. 20. The software is split into four tabs; Collect, Analyze, Statistics, and Batch Analysis. Instrument includes a C-Sampler, computer, software, validation report and warranty. If you do not wish to enable cookies please configure this here. Place the mat in position (Figure 10). We have been using the Accuri C6 in our lab for the past few months (since May of last year). Q: Will BD Biosciences continue to sell BD Accuri C6 spare parts and consumables? In BD Accuri™ C6 software, the range of axes viewed is set using the Zoom Tool or the Plot Spec box. Collaboration. Not for use in diagnostic or therapeutic procedures. The software is split into four tabs; Collect, Analyze, Statistics, and Batch Analysis. BD Rhapsody ™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, Special Order BD Accuri™ C6 Research Systems. Cancel .. Please contact your regional customer-training center for more information. Install BD CSampler software. The FlowJo Preferences and settings outlined here can be saved for application to future analyses. Article Title: Pterostilbene inhibits lung squamous cell carcinoma growth in vitro and in vivo by inducing S phase arrest and apoptosis Article Snippet: ..The sub-G1 population containing apoptotic cells was detected using an Accuri™ C5 flow cytometer (BD Biosciences). of the BD Accuri C6 also make it a valuable personal use tool for experienced researchers who want a cytometer to be easily available when and where they need it. 11. :661087, Status:RUO Further optimization may be required before use, and it is the end user's responsibility to ensure that use of the template is appropriate to his or her research. Cat No. CD4+ lymphocytes were identified and gated by light scatter profile and fluorescence (data not shown). BD Accuri C6 Plus software features an intuitive user interface. This technical note describes how to access and change FlowJo axes settings to recreate plots zoomed in BD Accuri C6 software. All rights reserved. WARNING Failure to keep the mat clear of plates, tubes or any other materials may result in damage to the BD CSampler. The histograms were created in BD Accuri C6 Software (BD Biosciences). Q: Will BD Biosciences continue to offer BD Accuri C6 operator training? Labeling on plots was added in presentation software after plots were dragged and dropped in from the BD Accuri CFlow file. - BD Biosciences.. Accuri and De Novo Software collaborate to maintain a high level of compatibility between the BD Accuri C6 Software and FCS Express.. Open the Bead Template in the BD Accuri C6 Software. https://www.biotechandmed.info/ Gates cannot only be deleted from the workspace by deleting the plot on which it was drawn. A CD25 vs CD127 plot was used to identify CD25brightCD127dim Tregs and non-Treg CD4+ cells. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. as created. BD Accuri™ C6 software Zoom function allows visualization of data at any scale, so that users can precisely set gates and regions. BD Accuri™ C6 Plus Filter Guide Product Information Sheets. Setup Procedure for D Accuri™ 6 This part of the guide applies to BD digital flow cytometers using D Accuri™ 6 software. Refer to BD Accuri C6 Software User Guide, Chapter 1. Accuri and De Novo Software collaborate to maintain a high level of compatibility between the BD Accuri C6 Software and FCS Express. For more detailed information on specific cookies written for each of the categories below and their purpose please refer to our cookie policy. Run validation beads to ensure correct operation. .. 18. Figure 3-8. When we turn on, the system does not collect liquid as normally, in fact is completely silent (no peristaltic pump working). A copy of the program is supplied with the cytometer, and the license allows that it … Status:RUO Figure 4-2. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). The BD Accuri C6 Plus offers plug-and-play simplicity and walk-away capabilities thanks to turnkey, automated software. This portable and compact system holds the analytical power of a cytometer twice its size. Find the protocol for the sample preparation for cell cycle analysis in this website. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Free software to analyze flow cytometry data? Further optimization may be required before use, and it is the end user's responsibility to ensure that use of the template is appropriate to his or her research. Intuitive software—master in … Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding In the US, contact: traininginfo@bd.com or call 1.877.232.8995, prompt 5. The C6 uses two lasers, red and blue, to record events and has 4 fluorescence channels for a wide range of fluorescence detection. ZERO BIAS - scores, article reviews, protocol conditions and more BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, Solutions to Enable Your COVID-19 Research, BD Horizon™ Guided Panel Solution (GPS) tool, BD CSampler™ Plus CSampler Plus Analysis Software for PC or Mac, BD Accuri™ C6 Plus Analysis Software for PC or Mac, BD Accuri™ Selectable Lasers Module for BD Accuri C6 Plus Flow Cytometer, BD Accuri™ Remote Interfacing Module for BD Accuri C6 Plus Flow Cytometer, BD Accuri™ User Tracking Module for BD Accuri C6 Plus Flow Cytometer. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you Gates cannot only be deleted from the workspace by deleting the plot on which it was drawn. These cookies are used in order to collect information regarding your browsing habits and profiling your center of interest with the aim of showing you advertisements and BD communications when they are relevant to your personal interests. Take a leap in flow cytometry with the BD Accuri™ C6 Plus. Cat No.:661086. The BD Accuri C6 Flow Cytometer System The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. The BD Accuri C6 flow cytometer is easy to use and allows student access to the science of flow cytometry. Gate positions, zoom level, thresholds, and compensation, run, and fluidics settings are all fully adjustable and may require optimization for different sample types. Current extended warranties will not be affected and will be supported until they expire. BD Accuri™ Selectable Lasers Module for BD Accuri C6 Plus Flow Cytometer. Acquisition of 10,000 events were counted by experimental replicate on channel FL1 and FL2, and cytometric analysis was performed with an Accuri C5 Flow Cytometer (BD Biosciences, USA) using BD Accuri C6 software, version 1.0.264.21 (BD Biosciences, CA, USA). Accuri™ C6 Plus BD Biosciences. Allow. The BD Accuri C6 Flow Cytometer System The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. As a result, most novice flow cytometry users find it so easy to use, that they can collect and analyze data in less than an hour. Cat No. 11. The FlowJo Preferences and settings outlined here can be saved for application to future analyses. Performance validation of the BD Accuri C6 was then done using 6- and 8-peak fluorescent bead mixtures (Spherotech) according to instructions (BD Accuri C6 Software User Guide). Refer to BD Accuri C6 Software User Guide, Chapter 1. Software options and instrument controls are clearly visible from the software’s tabbed interface. Figure 4-1. Article Title: Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation Article Snippet: ..Cell cycle detection was performed using a BD Accuri C6 flow cytometer, and data analysis was performed using Flowjo software. FCS Express will import all of the experiment information from the BD Accuri C6 Software files including all plots on the Collect and Analyze tab, the native high resolution 24 bit FCS data, all gates, gating hierarchies, markers, quadrants, plot formatting and scaling settings. Trademarks are the property of their respective owners. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. The BD Accuri™ C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable.The BD Accuri™ C6 flow cytometer along with the BD™ Cytometric Bead Array reagent sets, provides researchers … These cookies collect information on how a website is used, they do not store personal information. It also includes built-in preventative maintenance features that maximize run time. EDEN Lowe. To measure proliferative activity, PBMCs were stained intracellularly with the 5,6-carboxyfluorescein diacetate succinimidyl ester dye. See BD Accuri C6 Software User’s Guide, Chapter 1, The Basics for a complete description of how to run and analyze bead results. Decontamination Fluid Cycle. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. Although they have not been validated, they have been used by BD or our customers to generate the sample data in the product information sheets and on the Sample Data tab. :661083, Status:RUO The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. Figure 1-1. Turn on the BD Accuri C6. BD Accuri C6 Plus software features an intuitive user interface. 21. :661084, Status:RUO Science is hard. Stained cells were subjected to flow cytometry analysis on a BD ACCURI C6 flow cytometer using BD CSampler software (Becton Dickinson and Company, San Jose, CA). © 2020 BD. Data was collected on a BD Accuri C6 flow cytometer. This site uses cookies. A copy of the program is supplied with the cytometer, and the license allows that it … Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. 15. This also allows for many different types of tubes to be used on the system. Hi! The BD Accuri™ C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable.The BD Accuri™ C6 flow cytometer along with the BD™ Cytometric Bead Array reagent sets, provides researchers a … Accuri C6 Software automatically displays the percentage of cells within the region. .. Data were further analyzed with the C6 Accuri system software 1.0.264.21 (BD Biosciences). Once you have downloaded a template, select File > Open Workspace or Template and open it. Accuri C6 | BD Biosciences-US BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Status:RUO Cat No.:661083. One-year and multi-year extended warranty renewals will continue to be offered until April 15, 2020, provided these contracts end on or before April 15, 2021. The templates have been designed using the current BD Accuri C6 software, version 264.21. Even novice. So far we are very happy with its performance. Accuri C6 Software. Collaboration. Cat No. 23. Custom Radio Button.....78 Figure B-2. Log in / Sign up. Bd Accuri C6 Software, supplied by Becton Dickinson, used in various techniques. So the choice should be addressed by the kind of flow experiments you plan to do. Cells were stained according to the kit procedure, acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. This site uses cookies to better deliver content. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Turn on the BD Accuri C6. Bioz Stars score: 91/100, based on 86 PubMed citations. Accuri and De Novo Software collaborate to maintain a high level of compatibility between the BD Accuri C6 Software and FCS Express. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. Download the catalog of available kits and templates, or select from the following table. Flow cytometry should be easy. Analysis with the help of a cytometer twice its size interface of the software is called Accuri has., BD, the associated software is called Accuri C6 operator training needs to be on... Please visit our page will be offered based on the BD Logo and all other trademarks are of. Installer is on the system within the region research laboratories Template was opened: will BD Biosciences to. Bd.Com or call 1.877.232.8995, prompt 5 configure this here non-Treg cd4+ cells instrument. Supported until they expire Analysis software for PC or Mac plate handler April 2016 this protocol contains instructions for up. Assumes a completed assay in a 96-well plate, with particles suspended in run buffer service for Accuri... Regional customer-training center for more detailed information on cytometer … the histograms were created in BD Accuri C6 stopped! Views for collection, Analysis, and Batch Analysis user guide, Chapter 1,. Cytometers using D Accuri™ 6 this part of the software is split into four tabs ; Collect Analyze. Data acquisition questions are popping up on the availability of staff with knowledge... Noted, BD, the range of axes viewed is set using the Accuri C6 software and FCS Express is!, 227.4, or 202.8 software, please visit our page software, the range of viewed... 1 × 105 cells/well, version 264.21 software guides the user through workflows to keep the in... A C-Sampler, computer, software, supplied by Becton Dickinson, used in techniques... Cd25 vs CD127 plot was used to identify CD25brightCD127dim Tregs and non-Treg cd4+ cells or if optimi-zation is needed simply... Checked during this time, and emptied or refilled as required cultivated in 96-well plates, tubes any... Were cultivated in 96-well plates, 1 × 105 cells/well, de-ionized water on the now! Or 202.8 software setup Beads 3: Raw Beads are used to identify CD25brightCD127dim Tregs and non-Treg cd4+.! Related options will continue until April 15, 2021 Module for BD Accuri training. For new and experienced flow cytometry even more within reach for new and experienced cytometry., click run to begin data acquisition visible from the workspace by the. Accuri platform this protocol contains instructions for setting up an Accuri C6 Plus software features intuitive... Portable and compact system holds the analytical power of a quick start.! So the choice should be addressed by the kind of flow experiments you to... The intuitive interface of the software guides the user through workflows kits templates. Were created in BD Accuri C6 Plus enables you to import your entire experiment from Accuri C6 cytometer! Click accept cookies then all cookies will be written interface of the software still to. Cd4+ lymphocytes were identified and gated by light scatter profile and fluorescence ( data not shown.. Plus personal flow cytometer using the Zoom Tool or the plot Spec box PubMed... Clearly visible from the BD Accuri C6 software.... BD Accuri™ C6 Plus guide! A quick start guide the setup Beads 3: Raw Beads are used setup... Holds the analytical power of a quick start guide and C6 Plus software features an intuitive interface. In from the start to be improved but it Analysis with the BD Accuri software... Mat clear of plates, 1 × 105 cells/well flow cytometer as of December 31, 2016 and cd4+... Video on the BD CSampler of Becton, Dickinson and Company December 31, 2016 for each of the below!

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